Nucloside phosphoryalse (NP)

Famaritana fohy:

Momba ny nucleoside phosphoryalse

Misy karazany 3 ny vokatra anzima NP (Laharana toy ny ES-NP-101~ ES-NP-103) novolavolain'ny SyncoZymes.ES-NP-101 dia purine nucleoside phosphorylase, ES-NP-102 ary ES-NP-103 dia pyrimidine nucleoside phosphorylase.Ny nucleosidephosphorylase dia mety manimba ny nucleosides ho fototra sy pentose phosphate.Ny nucleoside phosphorylase dia azo zaraina ho purine nucleoside phosphorylase sy pyrimidine nucleoside phosphorylase araka ny tian'ny base nucleoside.Ny purine nucleoside phosphorylase dia afaka mamadika ny adenosine ho adenine, inosine ho hypoxanthine, guanosine ho guanine, ary mamokatra ribose phosphate amin'ny fotoana iray ihany.Ny pyrimidine nucleoside phosphorylase dia afaka mamadika ny uridine ho uracil ary mamokatra ribose phosphate.

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Product Detail

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Nucloside phosphoryalse karazana fanehoan-kevitra:

Product Information:

anzima	Code Product	famaritana
Enzyme vovoka	ES-NP-101~ ES-NP-103	andiany 3 Nuclosidephosphoryalse, 50 mg isaky ny entana 3 * 50mg / entana, na zavatra hafa

Fomba fitiliana ny fanehoan-kevitra Nucloside phosphoryalse:

Ity rafitra fanehoan-kevitra manaraka ity dia azo ampiasaina ho fanondroana:
1-10 mg/ml substrate
10 mg/ml Enzyme
100 mM Phosphate buffer (pH7.0)
Atsofohy amin'ny fanozongozonana hafanana tsy tapaka (ohatra, 30oC, 150rpm) mandritra ny 12-24 ora ny fanehoan-kevitra.Centrifuge ny fangaro tsirairay mba hanendasany proteinina, izy ireo ny supernatant azo ampiasaina amin'ny TLC, HPLC na GC fanadihadiana.